Detailed information of TA system TA160188

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 122081..122334
• Replicon: plasmid pKP20194d-p2
• Accession: NZ_CP054734
• Organism: Klebsiella pneumoniae strain KP20194d

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HUS06_RS28685
• Protein ID: WP_001312851.1
• Coordinates: 122185..122334 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	122081..122140 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS06_RS28645	117463..117807	-	345	Protein_143	IS6-like element IS26 family transposase	-
HUS06_RS28650	117859..118563	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HUS06_RS28655	118627..118788	+	162	Protein_145	DNA helicase	-
HUS06_RS28660	118808..119554	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HUS06_RS28665	119609..120169	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HUS06_RS28670	120301..120501	+	201	WP_015059022.1	hypothetical protein	-
HUS06_RS28675	120887..121486	+	600	WP_032083981.1	hypothetical protein	-
HUS06_RS28680	121650..121880	+	231	WP_001736714.1	hypothetical protein	-
-	122081..122140	-	60	NuclAT_1	-	Antitoxin
-	122081..122140	-	60	NuclAT_1	-	Antitoxin
-	122081..122140	-	60	NuclAT_1	-	Antitoxin
-	122081..122140	-	60	NuclAT_1	-	Antitoxin
HUS06_RS28685	122185..122334	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUS06_RS28690	122618..122866	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaSHV-12 / blaKPC-2	-	1..123177	123177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T160188 WP_001312851.1 NZ_CP054734:122185-122334 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T160188 NZ_CP070338:2645278-2645691 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT160188 NZ_CP054734:c122140-122081 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin