Detailed information of TA system TA160185

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24454..24723
• Replicon: plasmid pKP20194d-p2
• Accession: NZ_CP054734
• Organism: Klebsiella pneumoniae strain KP20194d

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HUS06_RS28085
• Protein ID: WP_001312861.1
• Coordinates: 24565..24723 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	24454..24519 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS06_RS28060	20164..20691	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HUS06_RS28065	20749..20982	+	234	WP_000006003.1	DUF905 family protein	-
HUS06_RS28070	21043..23066	+	2024	Protein_28	ParB/RepB/Spo0J family partition protein	-
HUS06_RS28075	23135..23569	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HUS06_RS28080	23566..24285	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	24297..24521	+	225	NuclAT_0	-	-
-	24297..24521	+	225	NuclAT_0	-	-
-	24297..24521	+	225	NuclAT_0	-	-
-	24297..24521	+	225	NuclAT_0	-	-
-	24454..24519	+	66	-	-	Antitoxin
HUS06_RS28085	24565..24723	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUS06_RS28090	24961..25338	-	378	Protein_32	hypothetical protein	-
HUS06_RS28095	25638..25934	+	297	WP_001272251.1	hypothetical protein	-
HUS06_RS28100	26045..26866	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HUS06_RS28105	27163..27810	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HUS06_RS28110	28087..28470	+	384	WP_000124981.1	relaxosome protein TraM	-
HUS06_RS28115	28751..29455	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaSHV-12 / blaKPC-2	-	1..123177	123177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T160185 WP_001312861.1 NZ_CP054734:24565-24723 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T160185 NZ_CP070338:c2182961-2182644 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT160185 NZ_CP054734:24454-24519 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin