Detailed information of TA system TA160181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 41512..42239
• Replicon: plasmid pKP20194d-p1
• Accession: NZ_CP054733
• Organism: Klebsiella pneumoniae strain KP20194d

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HUS06_RS27065
• Protein ID: WP_011251285.1
• Coordinates: 41928..42239 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUS06_RS27060
• Protein ID: WP_011251286.1
• Coordinates: 41512..41931 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS06_RS27030	36680..37150	-	471	WP_048333570.1	hypothetical protein	-
HUS06_RS27035	37343..38098	-	756	Protein_38	hypothetical protein	-
HUS06_RS27040	38694..39137	+	444	WP_048333569.1	hypothetical protein	-
HUS06_RS27045	39158..39946	+	789	WP_040217257.1	hypothetical protein	-
HUS06_RS27050	39960..40295	+	336	Protein_41	conjugal transfer protein TraI	-
HUS06_RS27055	40397..41365	-	969	WP_074428168.1	IS5 family transposase	-
HUS06_RS27060	41512..41931	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HUS06_RS27065	41928..42239	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HUS06_RS27070	42444..42902	-	459	Protein_45	DDE-type integrase/transposase/recombinase	-
HUS06_RS27075	43016..43713	+	698	Protein_46	IS1 family transposase	-
HUS06_RS27080	43729..44448	-	720	Protein_47	aldo/keto reductase	-
HUS06_RS27085	44445..44924	-	480	WP_004212799.1	hypothetical protein	-
HUS06_RS27090	44921..45106	-	186	WP_045145446.1	hypothetical protein	-
HUS06_RS27095	45110..45226	+	117	Protein_50	Tn3 family transposase	-
HUS06_RS27100	45509..45739	-	231	WP_011154571.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..194896	194896
	-	inside	IScluster/Tn	-	-	22562..43713	21151

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T160181 WP_011251285.1 NZ_CP054733:c42239-41928 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T160181 NZ_CP070338:c2005848-2005504 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT160181 WP_011251286.1 NZ_CP054733:c41931-41512 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT160181 NZ_CP070338:c2006090-2005842 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1