Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 41511..42238 Replicon plasmid pKP20194e-p1
Accession NZ_CP054727
Organism Klebsiella pneumoniae strain KP20194e

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag HUS07_RS27070 Protein ID WP_011251285.1
Coordinates 41927..42238 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUS07_RS27065 Protein ID WP_011251286.1
Coordinates 41511..41930 (-) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS07_RS27035 36679..37149 - 471 WP_048333570.1 hypothetical protein -
HUS07_RS27040 37342..38097 - 756 Protein_38 hypothetical protein -
HUS07_RS27045 38693..39136 + 444 WP_048333569.1 hypothetical protein -
HUS07_RS27050 39157..39945 + 789 WP_040217257.1 hypothetical protein -
HUS07_RS27055 39959..40294 + 336 Protein_41 conjugal transfer protein TraI -
HUS07_RS27060 40396..41364 - 969 WP_074428168.1 IS5 family transposase -
HUS07_RS27065 41511..41930 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
HUS07_RS27070 41927..42238 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
HUS07_RS27075 42443..42901 - 459 Protein_45 DDE-type integrase/transposase/recombinase -
HUS07_RS27080 43015..43712 + 698 Protein_46 IS1 family transposase -
HUS07_RS27085 43728..44447 - 720 Protein_47 aldo/keto reductase -
HUS07_RS27090 44444..44923 - 480 WP_004212799.1 hypothetical protein -
HUS07_RS27095 44920..45105 - 186 WP_045145446.1 hypothetical protein -
HUS07_RS27100 45109..45225 + 117 Protein_50 Tn3 family transposase -
HUS07_RS27105 45508..45738 - 231 WP_011154571.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..195027 195027
- inside IScluster/Tn - - 22688..43712 21024


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T160151 WP_011251285.1 NZ_CP054727:c42238-41927 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T160151 NZ_CP070327:2323591-2323767 [Ferrovum myxofaciens]
ATGAACTCCAAGCAGATGAAGAAATGGCTAGAGCAACAGGGCGCGACTTTCCAGCCCGGTAAAGGCTCGCACCTGAAGGT
GTTTCTGAATGGCAAGCAGTCTTCACTCCCCATGCACGGTACCGCCGAACTTGGTAAAGGGCTGGAAGCCGCGATCAAAC
GACAGCTTGGTTTGTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT160151 WP_011251286.1 NZ_CP054727:c41930-41511 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT160151 NZ_CP070327:2323781-2324200 [Ferrovum myxofaciens]
ATGATGTTCGACTACCCCGTGACCTTGACCCCCGATGACGGCACCATGCTGGTTACATTTGCCGATGTGCCCGAAGCCAT
CACCTTCGGTGCCGATGAGGATGAAGCCCTGCTGAACGCAATCGATGCGCTGGAAACGGGCTTGTCGTTTTATGTCGATG
CTCGCAAATCGCTACCCGTCGCCAGTCAGCCGGCGGCTGGACAAAAGACCGTGCGGCCATCGGCGCTGGAATGTGCCAAG
CTGGGCATTTACCAAGCGATGACCGATCAGGGAATCAAGAAGGCGGAACTCGCCCGCCGCTTGGGATGGCATATGCCGCA
AGTTGATCGCTTGTTCGATTTGCGTCATGCTTCAAAACTCGACCAGATCGAGGCTGCTGCCAACGTGCTCGGCAAACACG
TTTACGTGCAGATTGCCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References