Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3314343..3315070 Replicon chromosome
Accession NZ_CP054665
Organism Escherichia coli strain IGC_EcoliInv_1.1

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HUR94_RS16360 Protein ID WP_000550189.1
Coordinates 3314343..3314657 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUR94_RS16365 Protein ID WP_000560266.1
Coordinates 3314654..3315070 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUR94_RS16340 (3310501) 3310501..3311487 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
HUR94_RS16345 (3311566) 3311566..3312258 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HUR94_RS16350 (3312335) 3312335..3312838 - 504 WP_001333820.1 M48 family metallopeptidase -
HUR94_RS16355 (3312923) 3312923..3314059 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HUR94_RS16360 (3314343) 3314343..3314657 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HUR94_RS16365 (3314654) 3314654..3315070 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HUR94_RS16370 (3315115) 3315115..3317133 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HUR94_RS16375 (3317559) 3317559..3319910 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T160062 WP_000550189.1 NZ_CP054665:3314343-3314657 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T160062 NZ_CP070287:1058896-1059048 [Escherichia albertii]
ATGCTAACAAAATATGCCCTTGTGGCAATCATAGTGCTGTGTTGTACAGTGCTGGGATTCACGCTGATGGTAGGCGACTC
GTTGTGTGAGTTGAGTATCAGAGAACGTGGTATGGAGTTTAAGGCAGTTCTCGCTTACGAATCGAAGAAGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT160062 WP_000560266.1 NZ_CP054665:3314654-3315070 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT160062 NZ_CP070287:c1058854-1058802 [Escherichia albertii]
TTAGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References