Detailed information of TA system TA160027

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42778..43047
• Replicon: plasmid unnamed1
• Accession: NZ_CP054663
• Organism: Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: HUR93_RS25940
• Protein ID: WP_001323520.1
• Coordinates: 42931..43047 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	42778..42843 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR93_RS25900	37868..38098	+	231	WP_001380705.1	hypothetical protein	-
HUR93_RS25905	38571..39098	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
HUR93_RS25910	39154..39387	+	234	WP_000006004.1	DUF905 domain-containing protein	-
HUR93_RS25915	39446..41404	+	1959	WP_061892140.1	ParB/RepB/Spo0J family partition protein	-
HUR93_RS25920	41459..41893	+	435	WP_061892139.1	conjugation system SOS inhibitor PsiB	-
HUR93_RS25925	41890..42652	+	763	Protein_52	plasmid SOS inhibition protein A	-
HUR93_RS25930	42621..42809	-	189	WP_001299721.1	hypothetical protein	-
-	42621..42845	+	225	NuclAT_0	-	-
-	42621..42845	+	225	NuclAT_0	-	-
-	42621..42845	+	225	NuclAT_0	-	-
-	42621..42845	+	225	NuclAT_0	-	-
-	42778..42843	+	66	-	-	Antitoxin
HUR93_RS25935	42831..42980	+	150	Protein_54	plasmid maintenance protein Mok	-
HUR93_RS25940	42931..43047	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUR93_RS25945	43267..43497	+	231	WP_001426396.1	hypothetical protein	-
HUR93_RS25950	43495..43668	-	174	Protein_57	hypothetical protein	-
HUR93_RS25955	43738..43944	+	207	WP_000547974.1	hypothetical protein	-
HUR93_RS25960	43969..44256	+	288	WP_000107535.1	hypothetical protein	-
HUR93_RS25965	44376..45197	+	822	WP_001234469.1	DUF932 domain-containing protein	-
HUR93_RS25970	45494..46084	-	591	WP_165850686.1	transglycosylase SLT domain-containing protein	-
HUR93_RS25975	46419..46802	+	384	WP_046201845.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUR93_RS25980	46998..47669	+	672	WP_237897983.1	conjugal transfer transcriptional regulator TraJ	-
HUR93_RS25985	47806..48033	+	228	WP_000089265.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..108557	108557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T160027 WP_001323520.1 NZ_CP054663:42931-43047 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T160027 NZ_CP070281:3431474-3431692 [Escherichia albertii]
ATGTCCGATAAACCTTTAACAAAAACCGATTATTTGATGCGTCTGCGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
TATCGAGAAAAATAAATATGAATTATCAGATAATGAATTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTTGCAGAGC
TGACTATGAATAAGCTATATGACAAGATCCCTTCCTCAGTGTGGAAATTCATTCGGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT160027 NZ_CP054663:42778-42843 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin