Detailed information of TA system TA160025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5012011..5012613
• Replicon: chromosome
• Accession: NZ_CP054662
• Organism: Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HUR93_RS24400
• Protein ID: WP_000897305.1
• Coordinates: 5012302..5012613 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUR93_RS24395
• Protein ID: WP_000356397.1
• Coordinates: 5012011..5012301 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR93_RS24370 (5007937)	5007937..5008839	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HUR93_RS24375 (5008836)	5008836..5009471	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HUR93_RS24380 (5009468)	5009468..5010397	+	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
HUR93_RS24385 (5010727)	5010727..5010969	-	243	WP_001086388.1	protein YiiF	-
HUR93_RS24390 (5011188)	5011188..5011406	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HUR93_RS24395 (5012011)	5012011..5012301	-	291	WP_000356397.1	NadS family protein	Antitoxin
HUR93_RS24400 (5012302)	5012302..5012613	-	312	WP_000897305.1	hypothetical protein	Toxin
HUR93_RS24405 (5012842)	5012842..5013750	+	909	WP_001162704.1	alpha/beta hydrolase	-
HUR93_RS24410 (5013814)	5013814..5014755	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
HUR93_RS24415 (5014800)	5014800..5015237	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HUR93_RS24420 (5015234)	5015234..5016106	-	873	WP_061892615.1	virulence factor BrkB family protein	-
HUR93_RS24425 (5016100)	5016100..5016699	-	600	WP_001295269.1	glucose-1-phosphatase	-
HUR93_RS24430 (5016798)	5016798..5017583	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T160025 WP_000897305.1 NZ_CP054662:c5012613-5012302 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T160025 NZ_CP070281:2354028-2354414 [Escherichia albertii]
ATGATTTGGGTCAGTGCACAGGAGGTCATTGCCTTCCACGATCGAATTTTGCAACGTTTTCCCGGTGTGGCGGGCCTGAC
TGACCCCGGCAGAGCACAGGCGTTGATTTATCGCGTGCAGAATCGTGTTCACTACGAGGGCGTTACTGACCTGTTTGAAC
TGGCAGCAACCTACTGGGTTGCCATCGCTCGCGGTCACATCTTTCATGATGGTAATAAACGTACCGCATTTTTCGTTACT
ATGACCTTTCTTTGGCGCAATGGCATCCGTATCCGTGACGTTGATAACTCGCTGGAGAACCTGACTGTCGAAGCGGCTAC
CGGAGAAAAAACGGTCGGTCAATTAGCGCGATGTTTACGTGAACGAGTGGATAGTTCCGGGAATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT160025 WP_000356397.1 NZ_CP054662:c5012301-5012011 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT160025 NZ_CP070281:2353810-2354031 [Escherichia albertii]
ATGCAAACTTACACTTCCACACAAGCCAGAGCCAATATTGCCACTGTACTGGATACCGCGTTGAATGGTGAGCCAGTTGA
AATCACCCGTCGCGACGGCAGCTCAGCCGTGCTCATTAGTAAAGCCGCATTCGAGGCGTATCAAAATGCGAAACTGGACG
CGGAATTTAATGCCATCATGCAACGTCATGAACACACAATTGAAGCGTTGACTGATCGATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7