Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4091619..4092160 Replicon chromosome
Accession NZ_CP054662
Organism Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag HUR93_RS20245 Protein ID WP_000615982.1
Coordinates 4091882..4092160 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUR93_RS20240 Protein ID WP_000729704.1
Coordinates 4091619..4091879 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUR93_RS20215 (4087402) 4087402..4088187 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
HUR93_RS20220 (4088159) 4088159..4089871 + 1713 Protein_3961 flagellar biosynthesis protein FlhA -
HUR93_RS20225 (4089976) 4089976..4090254 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUR93_RS20230 (4090247) 4090247..4090603 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUR93_RS20235 (4090660) 4090660..4091433 - 774 WP_000543899.1 C40 family peptidase -
HUR93_RS20240 (4091619) 4091619..4091879 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUR93_RS20245 (4091882) 4091882..4092160 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUR93_RS20250 (4092316) 4092316..4093056 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HUR93_RS20255 (4093027) 4093027..4093794 - 768 WP_000333380.1 class II glutamine amidotransferase -
HUR93_RS20260 (4094000) 4094000..4094578 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T160021 WP_000615982.1 NZ_CP054662:4091882-4092160 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T160021 NZ_CP070281:612734-613048 [Escherichia albertii]
ATGCACCTGATCACTCAAAAAGCATTGAAAGATGCAGCGGAAAAATATCCGCAGCATAAAACGGAGTTGGTGGCACTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCTGTGTTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCACAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT160021 WP_000729704.1 NZ_CP054662:4091619-4091879 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT160021 NZ_CP070281:613045-613461 [Escherichia albertii]
ATGATTGCGATTGCCGATATCTTGCAAGCAGGAGAAAAGCTAACCGATGTGGCACCTTTTCTGGCGGGTATCCAGAGCGA
AGAACAGTATGCCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCGATGGTTCAAGCCATGCCTGGCGGT
GTTGCTGTGATTCGTACCCTGATGGATCAATACGGCTTAACCCTTTCCGATCTTCCAGAAATTGGCAGTAAATCCATGGT
ATCACGCGTTTTGAACGGGAAGAGAAAATTAACGCTGGAACACGCTAAAAAACTGGCAGCGCGATTCGGCATTTCCCCTG
CGTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References