Detailed information of TA system TA160013

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2291597..2291968
• Replicon: chromosome
• Accession: NZ_CP054662
• Organism: Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: D6I8D3
• Locus tag: HUR93_RS11235
• Protein ID: WP_001307771.1
• Coordinates: 2291774..2291968 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2291597..2291775 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR93_RS11195 (2287039)	2287039..2287695	-	657	WP_000011652.1	carbon-nitrogen hydrolase family protein YobB	-
HUR93_RS11200 (2287797)	2287797..2288027	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
HUR93_RS11205 (2288166)	2288166..2288540	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
HUR93_RS11210 (2288544)	2288544..2289416	+	873	WP_061892384.1	copper homeostasis membrane protein CopD	-
HUR93_RS11215 (2289429)	2289429..2289770	+	342	WP_000976492.1	YebY family protein	-
HUR93_RS11220 (2290163)	2290163..2291239	-	1077	WP_001189085.1	phage integrase Arm DNA-binding domain-containing protein	-
HUR93_RS11225 (2291205)	2291205..2291486	-	282	WP_001311878.1	excisionase	-
- (2291597)	2291597..2291775	+	179	NuclAT_1	-	Antitoxin
- (2291597)	2291597..2291775	+	179	NuclAT_1	-	Antitoxin
- (2291597)	2291597..2291775	+	179	NuclAT_1	-	Antitoxin
- (2291597)	2291597..2291775	+	179	NuclAT_1	-	Antitoxin
HUR93_RS11230 (2291593)	2291593..2291781	-	189	WP_024165285.1	DUF1187 family protein	-
HUR93_RS11235 (2291774)	2291774..2291968	-	195	WP_001307771.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HUR93_RS11240 (2292001)	2292001..2292681	-	681	WP_000186708.1	YqaJ viral recombinase family protein	-
HUR93_RS11245 (2292678)	2292678..2293463	-	786	WP_001484907.1	phage recombination protein Bet	-
HUR93_RS11250 (2293469)	2293469..2293765	-	297	WP_001484906.1	host-nuclease inhibitor Gam family protein	-
HUR93_RS11255 (2293929)	2293929..2295758	-	1830	Protein_2204	exonuclease VIII	-
HUR93_RS11260 (2295860)	2295860..2296135	-	276	WP_001484827.1	hypothetical protein	-
HUR93_RS11265 (2296210)	2296210..2296386	-	177	WP_061892283.1	YdaE family protein	-
HUR93_RS11270 (2296380)	2296380..2296601	-	222	WP_000560226.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2282388..2338189	55801

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 6986.91 Da
• Isoelectric Point: 9.6161

>T160013 WP_001307771.1 NZ_CP054662:c2291968-2291774 [Escherichia coli]
VRYTKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T160013 NZ_CP070232:c4207547-4207374 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT160013 NZ_CP054662:2291597-2291775 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCCGA
GAGCATTTTTTCGCATTCCGATTTGGTTAACTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7H9KGA4

Antitoxin