Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 675689..676488 Replicon chromosome
Accession NZ_CP054662
Organism Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag HUR93_RS03270 Protein ID WP_000347267.1
Coordinates 675689..676153 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HUR93_RS03275 Protein ID WP_001307405.1
Coordinates 676153..676488 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUR93_RS03240 (670690) 670690..671124 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HUR93_RS03245 (671142) 671142..672020 - 879 WP_061892540.1 PTS N-acetylgalactosamine transporter subunit IID -
HUR93_RS03250 (672010) 672010..672789 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HUR93_RS03255 (672800) 672800..673273 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HUR93_RS03260 (673296) 673296..674576 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HUR93_RS03265 (674825) 674825..675634 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HUR93_RS03270 (675689) 675689..676153 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HUR93_RS03275 (676153) 676153..676488 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HUR93_RS03280 (676637) 676637..678241 - 1605 WP_061892539.1 galactarate dehydratase -
HUR93_RS03285 (678616) 678616..679950 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HUR93_RS03290 (679966) 679966..680736 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T159997 WP_000347267.1 NZ_CP054662:c676153-675689 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T159997 NZ_CP070232:826008-826415 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT159997 WP_001307405.1 NZ_CP054662:c676488-676153 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT159997 NZ_CP070232:825761-826027 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References