Detailed information of TA system TA159990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5513075..5513670
• Replicon: chromosome
• Accession: NZ_CP054623
• Organism: Pseudomonas aeruginosa strain DL201330

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HUF09_RS25905
• Protein ID: WP_003117425.1
• Coordinates: 5513075..5513353 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUF09_RS25910
• Protein ID: WP_003099268.1
• Coordinates: 5513365..5513670 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUF09_RS25890	5508505..5509743	+	1239	WP_014603324.1	C69 family dipeptidase	-
HUF09_RS25895	5509805..5510452	+	648	WP_003095021.1	carbonate dehydratase	-
HUF09_RS25900	5510522..5512750	-	2229	WP_003113525.1	TonB-dependent receptor	-
HUF09_RS25905	5513075..5513353	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUF09_RS25910	5513365..5513670	+	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HUF09_RS25920	5514077..5515177	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HUF09_RS25925	5515218..5515802	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HUF09_RS25930	5515844..5516458	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HUF09_RS25935	5516575..5517516	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HUF09_RS25945	5517683..5518531	-	849	WP_003162406.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T159990 WP_003117425.1 NZ_CP054623:5513075-5513353 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T159990 NZ_CP070230:61613-62011 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAGCCCACCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AGCGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTTCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATAGTTGTGACTAACAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT159990 WP_003099268.1 NZ_CP054623:5513365-5513670 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT159990 NZ_CP070230:61386-61613 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTACCAGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGTA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5