Detailed information of TA system TA159958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 891134..891729
• Replicon: chromosome
• Accession: NZ_CP054591
• Organism: Pseudomonas aeruginosa strain CDN118

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HR116_RS03945
• Protein ID: WP_003113526.1
• Coordinates: 891134..891412 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HR116_RS03950
• Protein ID: WP_003113527.1
• Coordinates: 891424..891729 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR116_RS03930	886560..887798	+	1239	WP_033942304.1	C69 family dipeptidase	-
HR116_RS03935	887860..888507	+	648	WP_003095021.1	carbonate dehydratase	-
HR116_RS03940	888577..890805	-	2229	WP_033942306.1	TonB-dependent receptor	-
HR116_RS03945	891134..891412	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HR116_RS03950	891424..891729	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HR116_RS03960	892124..893224	-	1101	WP_033942321.1	redox-regulated ATPase YchF	-
HR116_RS03965	893265..893849	-	585	WP_033942324.1	aminoacyl-tRNA hydrolase	-
HR116_RS03970	893891..894505	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HR116_RS03975	894622..895563	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HR116_RS03985	895730..896578	-	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T159958 WP_003113526.1 NZ_CP054591:891134-891412 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T159958 NZ_CP070227:c3077421-3077170 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT159958 WP_003113527.1 NZ_CP054591:891424-891729 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT159958 NZ_CP070227:c3077173-3076919 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: V6ALY3