Detailed information of TA system TA159944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6107078..6107673
• Replicon: chromosome
• Accession: NZ_CP054581
• Organism: Pseudomonas aeruginosa strain YTSEY8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HUF04_RS29180
• Protein ID: WP_003117425.1
• Coordinates: 6107395..6107673 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HUF04_RS29175
• Protein ID: WP_003113527.1
• Coordinates: 6107078..6107383 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUF04_RS29150	6102191..6102481	-	291	WP_033992920.1	DUF5447 family protein	-
HUF04_RS29155	6102693..6102965	-	273	WP_003115921.1	hypothetical protein	-
HUF04_RS29160	6103495..6104592	+	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
HUF04_RS29165	6104595..6105644	+	1050	WP_003113529.1	serine/threonine protein kinase	-
HUF04_RS29170	6105641..6106714	+	1074	WP_173834565.1	serine/threonine protein kinase	-
HUF04_RS29175	6107078..6107383	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HUF04_RS29180	6107395..6107673	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUF04_RS29185	6108002..6110230	+	2229	WP_003099265.1	TonB-dependent receptor	-
HUF04_RS29190	6110300..6110947	-	648	WP_003095021.1	carbonate dehydratase	-
HUF04_RS29195	6111009..6112247	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T159944 WP_003117425.1 NZ_CP054581:c6107673-6107395 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T159944 NZ_CP070227:172242-172553 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT159944 WP_003113527.1 NZ_CP054581:c6107383-6107078 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT159944 NZ_CP070227:172554-172844 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5