Detailed information of TA system TA159936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5598281..5598849
• Replicon: chromosome
• Accession: NZ_CP054580
• Organism: Halomonas titanicae strain GPM3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1G8P1L0
• Locus tag: FX987_RS25955
• Protein ID: WP_022522161.1
• Coordinates: 5598281..5598559 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FX987_RS25960
• Protein ID: WP_022522160.1
• Coordinates: 5598568..5598849 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX987_RS25940	5593950..5595242	-	1293	WP_022522164.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
FX987_RS25945	5595266..5596435	-	1170	WP_053856867.1	acetyltransferase	-
FX987_RS25950	5596590..5598245	+	1656	WP_022522162.1	cyclic peptide export ABC transporter	-
FX987_RS25955	5598281..5598559	+	279	WP_022522161.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FX987_RS25960	5598568..5598849	+	282	WP_022522160.1	HigA family addiction module antidote protein	Antitoxin
FX987_RS25965	5599184..5600383	-	1200	WP_022522159.1	hypothetical protein	-
FX987_RS25970	5600380..5603772	-	3393	WP_022522158.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10504.94 Da
• Isoelectric Point: 10.0038

>T159936 WP_022522161.1 NZ_CP054580:5598281-5598559 [Halomonas titanicae]
MIKNFRHKGLRKFFETGSTSGIQAKHATKLQIQLTALDSAKGPEDMNAPGWRLHPLSHKLKGHWSITVNGNWRLTFAFEG
EDAILVDYQDYH

Nucleotide

• Length: 279 bp

>T159936 NZ_CP070172:3494571-3495893 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10210.68 Da
• Isoelectric Point: 8.6800

>AT159936 WP_022522160.1 NZ_CP054580:5598568-5598849 [Halomonas titanicae]
MNRMHNPAHPGTVLREYIGDISVTQAAQQLGVTRTALSRILNGNAGISADMALRLEQALGTSAEMWLEMQLKYELWQASQ
RPRAQVTPLHAAG

Nucleotide

• Length: 282 bp

>AT159936 NZ_CP070172:3494305-3494571 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A1G8P1L0