Detailed information of TA system TA159932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5173414..5173990
• Replicon: chromosome
• Accession: NZ_CP054580
• Organism: Halomonas titanicae strain GPM3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1G8HXM7
• Locus tag: FX987_RS24135
• Protein ID: WP_053856897.1
• Coordinates: 5173414..5173692 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: FX987_RS24140
• Protein ID: WP_022520034.1
• Coordinates: 5173703..5173990 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX987_RS24100	5168458..5169297	+	840	WP_022520042.1	hypothetical protein	-
FX987_RS24105	5169326..5170135	+	810	WP_022520041.1	hypothetical protein	-
FX987_RS24110	5170410..5170640	+	231	Protein_4741	MBL fold metallo-hydrolase	-
FX987_RS24115	5170730..5171575	+	846	WP_022520039.1	EamA family transporter	-
FX987_RS24120	5171653..5171931	+	279	WP_022520038.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FX987_RS24125	5171931..5172224	+	294	WP_022520037.1	HigA family addiction module antidote protein	-
FX987_RS24130	5172343..5173305	+	963	WP_022520035.1	ribonuclease Z	-
FX987_RS24135	5173414..5173692	+	279	WP_053856897.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FX987_RS24140	5173703..5173990	+	288	WP_022520034.1	HigA family addiction module antidote protein	Antitoxin
FX987_RS24145	5174097..5174516	-	420	WP_022520033.1	hypothetical protein	-
FX987_RS24150	5174784..5175362	+	579	WP_022520031.1	class I SAM-dependent methyltransferase	-
FX987_RS24155	5175632..5176153	-	522	WP_022520030.1	NAD(P)H-dependent oxidoreductase	-
FX987_RS24160	5176819..5177472	+	654	WP_022520029.1	DsbA family oxidoreductase	-
FX987_RS24165	5177572..5178765	-	1194	WP_022520028.1	homoserine O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5160756..5176153	15397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-86)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10331.78 Da
• Isoelectric Point: 10.6139

>T159932 WP_053856897.1 NZ_CP054580:5173414-5173692 [Halomonas titanicae]
MIRSFKHKGLAKFFKSGSTAGIQAAHAKRLRLILGRLNAASDVNDMDLPGLRLHELSGKRSGIWSVTVSGNWRVTFRFEN
GDAEIVNYEDYH

Nucleotide

• Length: 279 bp

>T159932 NZ_CP070172:1482131-1482472 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAATGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACAGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10442.08 Da
• Isoelectric Point: 7.3596

>AT159932 WP_022520034.1 NZ_CP054580:5173703-5173990 [Halomonas titanicae]
MLMHNPPHPGEVLRELCLEPLGLTVTAAAEALGVSRKTLSAVLNGKAGISPEMAVRLSIAFDTSAESWLNQQSQYELWHA
EQHRKELKVKRLVAA

Nucleotide

• Length: 288 bp

>AT159932 NZ_CP070172:1481769-1482110 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGGCACCTTGCTTTGGCGTTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATTTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCGCATTTTATCAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Structures

Toxin

• AlphaFold DB: A0A1G8HXM7