Detailed information of TA system TA159886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3718710..3719509
• Replicon: chromosome
• Accession: NZ_CP054556
• Organism: Escherichia coli strain LWY24

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HT750_RS18005
• Protein ID: WP_000347273.1
• Coordinates: 3718710..3719174 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: HT750_RS18010
• Protein ID: WP_001308975.1
• Coordinates: 3719174..3719509 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HT750_RS17975	3713711..3714145	-	435	WP_000948834.1	PTS sugar transporter subunit IIA	-
HT750_RS17980	3714163..3715041	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HT750_RS17985	3715031..3715810	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HT750_RS17990	3715821..3716294	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HT750_RS17995	3716317..3717597	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HT750_RS18000	3717846..3718655	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HT750_RS18005	3718710..3719174	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HT750_RS18010	3719174..3719509	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HT750_RS18015	3719658..3721229	-	1572	WP_001273756.1	galactarate dehydratase	-
HT750_RS18020	3721604..3722938	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HT750_RS18025	3722954..3723724	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T159886 WP_000347273.1 NZ_CP054556:c3719174-3718710 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T159886 NZ_CP070162:c3377940-3377629 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGCGCGCGCAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAAGCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT159886 WP_001308975.1 NZ_CP054556:c3719509-3719174 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT159886 NZ_CP070162:c3377632-3377213 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08