Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1174827..1175329 Replicon chromosome
Accession NZ_CP054556
Organism Escherichia coli strain LWY24

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HT750_RS05910 Protein ID WP_000767829.1
Coordinates 1174827..1175081 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HT750_RS05915 Protein ID WP_001259255.1
Coordinates 1175078..1175329 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HT750_RS05880 1169842..1170069 - 228 WP_000985278.1 sulfurtransferase TusA family protein -
HT750_RS05885 1170083..1171141 - 1059 WP_174617244.1 transport protein YeeE -
HT750_RS05890 1171320..1172678 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HT750_RS05895 1172668..1172730 - 63 WP_010723108.1 membrane protein YoeI -
HT750_RS05900 1172945..1173874 - 930 WP_000803351.1 LysR family transcriptional regulator -
HT750_RS05905 1173920..1174744 - 825 WP_000754758.1 SDR family oxidoreductase -
HT750_RS05910 1174827..1175081 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HT750_RS05915 1175078..1175329 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HT750_RS05920 1175612..1175662 + 51 WP_001364200.1 his operon leader peptide -
HT750_RS05925 1175808..1176707 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HT750_RS05930 1176713..1178017 + 1305 WP_021293346.1 histidinol dehydrogenase -
HT750_RS05935 1178014..1179084 + 1071 WP_001410397.1 histidinol-phosphate transaminase -
HT750_RS05940 1179084..1180151 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T159874 WP_000767829.1 NZ_CP054556:c1175081-1174827 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T159874 NZ_CP070162:339771-340145 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT159874 WP_001259255.1 NZ_CP054556:c1175329-1175078 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT159874 NZ_CP070162:339313-339681 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACTCATCCCGATGACAACAACAGCCACCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAGCTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTTACCTGCAAAGCCGATACCCTCGGCAGTCG
AGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References