Detailed information of TA system TA159779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5360795..5361390
• Replicon: chromosome
• Accession: NZ_CP054472
• Organism: Pseudomonas aeruginosa strain PAAK088

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MS088_RS24835
• Protein ID: WP_003113526.1
• Coordinates: 5361112..5361390 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS088_RS24830
• Protein ID: WP_003099268.1
• Coordinates: 5360795..5361100 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS088_RS24795	5355935..5356783	+	849	WP_023115601.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
MS088_RS24805	5356950..5357891	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MS088_RS24810	5358008..5358622	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
MS088_RS24815	5358664..5359248	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MS088_RS24820	5359289..5360389	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MS088_RS24830	5360795..5361100	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
MS088_RS24835	5361112..5361390	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS088_RS24840	5361719..5363947	+	2229	WP_031633241.1	TonB-dependent receptor	-
MS088_RS24845	5364017..5364664	-	648	WP_003095021.1	carbonate dehydratase	-
MS088_RS24850	5364726..5365964	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T159779 WP_003113526.1 NZ_CP054472:c5361390-5361112 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T159779 NZ_CP070134:3440976-3441266 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT159779 WP_003099268.1 NZ_CP054472:c5361100-5360795 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT159779 NZ_CP070134:3440744-3440986 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: V6ALY3