Detailed information of TA system TA159719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3559544..3560201
• Replicon: chromosome
• Accession: NZ_CP054411
• Organism: Enterobacter hormaechei strain KCJ3K19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FCG87_RS16815
• Protein ID: WP_063978808.1
• Coordinates: 3559544..3559843 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0F0ZCT3
• Locus tag: FCG87_RS16820
• Protein ID: WP_022651701.1
• Coordinates: 3559854..3560201 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCG87_RS16805 (FCG87_016805)	3555921..3558113	+	2193	WP_045346161.1	type I secretion system permease/ATPase	-
FCG87_RS16810 (FCG87_016810)	3558094..3559293	+	1200	WP_045346162.1	HlyD family efflux transporter periplasmic adaptor subunit	-
FCG87_RS16815 (FCG87_016815)	3559544..3559843	+	300	WP_063978808.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FCG87_RS16820 (FCG87_016820)	3559854..3560201	+	348	WP_022651701.1	HigA family addiction module antitoxin	Antitoxin
FCG87_RS16825 (FCG87_016825)	3560277..3561458	-	1182	WP_022651702.1	PLP-dependent aminotransferase family protein	-
FCG87_RS16830 (FCG87_016830)	3561478..3562365	-	888	WP_032660046.1	LysR family transcriptional regulator	-
FCG87_RS16835 (FCG87_016835)	3562463..3563065	+	603	WP_048210584.1	short chain dehydrogenase	-
FCG87_RS16840 (FCG87_016840)	3563062..3563793	-	732	WP_137048338.1	class I SAM-dependent methyltransferase	-
FCG87_RS16845 (FCG87_016845)	3563868..3564866	-	999	WP_045346167.1	transketolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11606.25 Da
• Isoelectric Point: 9.6776

>T159719 WP_063978808.1 NZ_CP054411:3559544-3559843 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNIYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T159719 NZ_CP070115:2315992-2316375 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13096.09 Da
• Isoelectric Point: 9.5932

>AT159719 WP_022651701.1 NZ_CP054411:3559854-3560201 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVA

Nucleotide

• Length: 348 bp

>AT159719 NZ_CP070115:2315534-2315902 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F0ZCT3