Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 31732..32462 Replicon chromosome
Accession NZ_CP054411
Organism Enterobacter hormaechei strain KCJ3K19

Toxin (Protein)


Gene name higB Uniprot ID A0A837FIL2
Locus tag FCG87_RS00155 Protein ID WP_023302825.1
Coordinates 31732..32046 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A837FJX0
Locus tag FCG87_RS00160 Protein ID WP_015570115.1
Coordinates 32046..32462 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FCG87_RS00130 (FCG87_000130) 27399..28484 + 1086 Protein_25 cellulase family glycosylhydrolase -
FCG87_RS00135 (FCG87_000135) 28390..29574 - 1185 WP_048245027.1 multidrug efflux MFS transporter EmrD -
FCG87_RS00140 (FCG87_000140) 29750..30583 - 834 WP_023323922.1 DMT family transporter -
FCG87_RS00145 (FCG87_000145) 30646..31092 - 447 WP_003861064.1 GNAT family N-acetyltransferase -
FCG87_RS00155 (FCG87_000155) 31732..32046 + 315 WP_023302825.1 type II toxin-antitoxin system HigB family toxin Toxin
FCG87_RS00160 (FCG87_000160) 32046..32462 + 417 WP_015570115.1 helix-turn-helix domain-containing protein Antitoxin
FCG87_RS00165 (FCG87_000165) 32609..32707 + 99 WP_057979964.1 ilvB operon leader peptide IvbL -
FCG87_RS00170 (FCG87_000170) 32937..34625 + 1689 WP_003861056.1 acetolactate synthase large subunit -
FCG87_RS00175 (FCG87_000175) 34629..34916 + 288 WP_003861055.1 acetolactate synthase small subunit -
FCG87_RS00180 (FCG87_000180) 34999..35592 + 594 WP_003861054.1 transcriptional regulator UhpA -
FCG87_RS00185 (FCG87_000185) 35589..37094 + 1506 WP_045336028.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12415.51 Da        Isoelectric Point: 10.2825

>T159706 WP_023302825.1 NZ_CP054411:31732-32046 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T159706 NZ_CP070111:4416146-4416553 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15367.95 Da        Isoelectric Point: 5.7670

>AT159706 WP_015570115.1 NZ_CP054411:32046-32462 [Enterobacter hormaechei]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 417 bp

>AT159706 NZ_CP070111:4415899-4416165 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACATCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FIL2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FJX0

References