Detailed information of TA system TA159689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4749998..4750600
• Replicon: chromosome
• Accession: NZ_CP054407
• Organism: Escherichia coli strain KCJ3K291

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FR957_RS23200
• Protein ID: WP_000897305.1
• Coordinates: 4750289..4750600 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FR957_RS23195
• Protein ID: WP_000356397.1
• Coordinates: 4749998..4750288 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR957_RS23170 (4745924)	4745924..4746826	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FR957_RS23175 (4746823)	4746823..4747458	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FR957_RS23180 (4747455)	4747455..4748384	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FR957_RS23185 (4748714)	4748714..4748956	-	243	WP_001086388.1	protein YiiF	-
FR957_RS23190 (4749175)	4749175..4749393	-	219	WP_001297075.1	CopG family transcriptional regulator	-
FR957_RS23195 (4749998)	4749998..4750288	-	291	WP_000356397.1	NadS family protein	Antitoxin
FR957_RS23200 (4750289)	4750289..4750600	-	312	WP_000897305.1	hypothetical protein	Toxin
FR957_RS23205 (4750829)	4750829..4751737	+	909	WP_001162704.1	alpha/beta hydrolase	-
FR957_RS23210 (4751801)	4751801..4752742	-	942	WP_001395735.1	fatty acid biosynthesis protein FabY	-
FR957_RS23215 (4752787)	4752787..4753224	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
FR957_RS23220 (4753221)	4753221..4754093	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FR957_RS23225 (4754087)	4754087..4754686	-	600	WP_001295269.1	glucose-1-phosphatase	-
FR957_RS23230 (4754785)	4754785..4755570	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T159689 WP_000897305.1 NZ_CP054407:c4750600-4750289 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T159689 NZ_CP070111:726548-726650 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT159689 WP_000356397.1 NZ_CP054407:c4750288-4749998 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT159689 NZ_CP070111:c726688-726420 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7