Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3889850..3890391 Replicon chromosome
Accession NZ_CP054407
Organism Escherichia coli strain KCJ3K291

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FR957_RS19265 Protein ID WP_000615976.1
Coordinates 3890113..3890391 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FR957_RS19260 Protein ID WP_000729704.1
Coordinates 3889850..3890110 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR957_RS19235 (3884886) 3884886..3886025 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
FR957_RS19240 (3886009) 3886009..3888102 + 2094 WP_001140827.1 flagellar biosynthesis protein FlhA -
FR957_RS19245 (3888207) 3888207..3888485 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FR957_RS19250 (3888478) 3888478..3888834 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
FR957_RS19255 (3888891) 3888891..3889664 - 774 WP_000543895.1 C40 family peptidase -
FR957_RS19260 (3889850) 3889850..3890110 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FR957_RS19265 (3890113) 3890113..3890391 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FR957_RS19270 (3890547) 3890547..3891287 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
FR957_RS19275 (3891258) 3891258..3892025 - 768 WP_000333380.1 class II glutamine amidotransferase -
FR957_RS19280 (3892231) 3892231..3892809 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / hsiC1/vipB / hsiB1/vipA 3888478..3931838 43360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T159685 WP_000615976.1 NZ_CP054407:3890113-3890391 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T159685 NZ_CP070111:407752-408006 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT159685 WP_000729704.1 NZ_CP054407:3889850-3890110 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT159685 NZ_CP070111:407504-407755 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References