Detailed information of TA system TA159670

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2353168..2353527
• Replicon: chromosome
• Accession: NZ_CP054407
• Organism: Escherichia coli strain KCJ3K291

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: FR957_RS11680
• Protein ID: WP_001317028.1
• Coordinates: 2353333..2353527 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2353168..2353334 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR957_RS11655 (2349123)	2349123..2350496	+	1374	WP_000123739.1	ATP-dependent RNA helicase DbpA	-
FR957_RS11660 (2350625)	2350625..2351560	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FR957_RS11665 (2351612)	2351612..2352847	-	1236	WP_000040852.1	site-specific integrase	-
FR957_RS11670 (2352849)	2352849..2353064	-	216	WP_000079604.1	excisionase XisR	-
- (2353168)	2353168..2353334	+	167	NuclAT_1	-	Antitoxin
- (2353168)	2353168..2353334	+	167	NuclAT_1	-	Antitoxin
- (2353168)	2353168..2353334	+	167	NuclAT_1	-	Antitoxin
- (2353168)	2353168..2353334	+	167	NuclAT_1	-	Antitoxin
FR957_RS11675 (2353143)	2353143..2353340	-	198	WP_024170988.1	DUF1187 family protein	-
FR957_RS11680 (2353333)	2353333..2353527	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FR957_RS11685 (2353584)	2353584..2354393	-	810	WP_000166319.1	recombination protein RecT	-
FR957_RS11690 (2354386)	2354386..2356986	-	2601	WP_000105136.1	exodeoxyribonuclease VIII	-
FR957_RS11695 (2357088)	2357088..2357363	-	276	WP_024251429.1	hypothetical protein	-
FR957_RS11700 (2357438)	2357438..2357608	-	171	WP_001352098.1	YdaE family protein	-
FR957_RS11705 (2357608)	2357608..2357829	-	222	WP_000560224.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2351612..2401998	50386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T159670 WP_001317028.1 NZ_CP054407:c2353527-2353333 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T159670 NZ_CP070103:c4328847-4328596 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 167 bp

>AT159670 NZ_CP054407:2353168-2353334 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAATTTTACCTGAGAGCATTTTTTC
GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGG
TAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin