Detailed information of TA system TA159663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 610156..610955
• Replicon: chromosome
• Accession: NZ_CP054407
• Organism: Escherichia coli strain KCJ3K291

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: FR957_RS03005
• Protein ID: WP_000347253.1
• Coordinates: 610156..610620 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: FR957_RS03010
• Protein ID: WP_024251361.1
• Coordinates: 610620..610955 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR957_RS02975 (605157)	605157..605591	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
FR957_RS02980 (605609)	605609..606487	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
FR957_RS02985 (606477)	606477..607256	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
FR957_RS02990 (607267)	607267..607740	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
FR957_RS02995 (607763)	607763..609043	-	1281	WP_146939073.1	tagatose-bisphosphate aldolase subunit KbaZ	-
FR957_RS03000 (609292)	609292..610101	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
FR957_RS03005 (610156)	610156..610620	-	465	WP_000347253.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
FR957_RS03010 (610620)	610620..610955	-	336	WP_024251361.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
FR957_RS03015 (611104)	611104..612675	-	1572	WP_001273766.1	galactarate dehydratase	-
FR957_RS03020 (613050)	613050..614384	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
FR957_RS03025 (614400)	614400..615170	+	771	WP_001058226.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17764.14 Da
• Isoelectric Point: 9.4947

>T159663 WP_000347253.1 NZ_CP054407:c610620-610156 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEETH

Nucleotide

• Length: 465 bp

>T159663 NZ_CP070103:c3070729-3070343 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12348.90 Da
• Isoelectric Point: 4.8616

>AT159663 WP_024251361.1 NZ_CP054407:c610955-610620 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
SQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT159663 NZ_CP070103:c3070353-3070024 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA