Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3745162..3745759 Replicon chromosome
Accession NZ_CP054406
Organism Klebsiella pneumoniae strain KCJ3K293

Toxin (Protein)


Gene name higB Uniprot ID A0A9J6S5A1
Locus tag FOH49_RS20160 Protein ID WP_004893639.1
Coordinates 3745442..3745759 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag FOH49_RS20155 Protein ID WP_004142561.1
Coordinates 3745162..3745449 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOH49_RS20125 (FOH49_020155) 3741242..3741490 + 249 WP_032410665.1 DUF1158 domain-containing protein -
FOH49_RS20130 (FOH49_020160) 3741508..3741849 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
FOH49_RS20135 (FOH49_020165) 3741880..3742995 - 1116 WP_185967404.1 MBL fold metallo-hydrolase -
FOH49_RS20140 (FOH49_020170) 3743175..3743756 + 582 WP_032410661.1 TetR/AcrR family transcriptional regulator -
FOH49_RS20145 (FOH49_020175) 3743756..3744124 + 369 WP_032421096.1 MmcQ/YjbR family DNA-binding protein -
FOH49_RS20150 (FOH49_020180) 3744244..3744897 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
FOH49_RS20155 (FOH49_020185) 3745162..3745449 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
FOH49_RS20160 (FOH49_020190) 3745442..3745759 - 318 WP_004893639.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOH49_RS20165 (FOH49_020195) 3745944..3746987 - 1044 WP_032410657.1 DUF2157 domain-containing protein -
FOH49_RS20170 (FOH49_020200) 3747653..3748519 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
FOH49_RS20175 (FOH49_020205) 3748628..3750055 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12100.35 Da        Isoelectric Point: 11.2767

>T159656 WP_004893639.1 NZ_CP054406:c3745759-3745442 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T159656 NZ_CP070103:c1257076-1256969 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT159656 WP_004142561.1 NZ_CP054406:c3745449-3745162 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT159656 NZ_CP070103:1257133-1257190 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References