Detailed information of TA system TA159634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3745189..3745786
• Replicon: chromosome
• Accession: NZ_CP054403
• Organism: Klebsiella pneumoniae strain KCJ3K292

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: FOH48_RS20215
• Protein ID: WP_004893639.1
• Coordinates: 3745469..3745786 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: FOH48_RS20210
• Protein ID: WP_004142561.1
• Coordinates: 3745189..3745476 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH48_RS20180 (FOH48_020110)	3741269..3741517	+	249	WP_032410665.1	DUF1158 domain-containing protein	-
FOH48_RS20185 (FOH48_020115)	3741535..3741876	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
FOH48_RS20190 (FOH48_020120)	3741907..3743022	-	1116	WP_185967404.1	MBL fold metallo-hydrolase	-
FOH48_RS20195 (FOH48_020125)	3743202..3743783	+	582	WP_032410661.1	TetR/AcrR family transcriptional regulator	-
FOH48_RS20200 (FOH48_020130)	3743783..3744151	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
FOH48_RS20205 (FOH48_020135)	3744271..3744924	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
FOH48_RS20210 (FOH48_020140)	3745189..3745476	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
FOH48_RS20215 (FOH48_020145)	3745469..3745786	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOH48_RS20220 (FOH48_020150)	3745971..3747014	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
FOH48_RS20225 (FOH48_020155)	3747680..3748546	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
FOH48_RS20230 (FOH48_020160)	3748655..3750082	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T159634 WP_004893639.1 NZ_CP054403:c3745786-3745469 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T159634 NZ_CP070098:3606896-3607360 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT159634 WP_004142561.1 NZ_CP054403:c3745476-3745189 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT159634 NZ_CP070098:3606561-3606896 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1