Detailed information of TA system TA159620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 52956..53613
• Replicon: plasmid pKC148K
• Accession: NZ_CP054402
• Organism: Klebsiella pneumoniae strain KCJ3K292

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FOH48_RS01045
• Protein ID: WP_000270043.1
• Coordinates: 53263..53613 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOH48_RS01040
• Protein ID: WP_000124640.1
• Coordinates: 52956..53258 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH48_RS00995 (FOH48_000970)	48581..49009	+	429	WP_000591074.1	hypothetical protein	-
FOH48_RS01000 (FOH48_000975)	49066..49425	+	360	WP_000422768.1	hypothetical protein	-
FOH48_RS01005 (FOH48_000980)	49425..49871	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
FOH48_RS01010 (FOH48_000985)	49868..50386	+	519	WP_000210756.1	nitrite reductase	-
FOH48_RS01015 (FOH48_000990)	50386..50616	+	231	WP_000972663.1	hypothetical protein	-
FOH48_RS01020 (FOH48_000995)	50603..51460	+	858	WP_001167032.1	hypothetical protein	-
FOH48_RS01025 (FOH48_001000)	51691..52218	+	528	WP_001236377.1	thermonuclease family protein	-
FOH48_RS01030 (FOH48_001005)	52276..52548	+	273	WP_001043047.1	HU family DNA-binding protein	-
FOH48_RS01035 (FOH48_001010)	52636..52929	+	294	WP_001239997.1	chromosome segregation protein ParM	-
FOH48_RS01040 (FOH48_001015)	52956..53258	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FOH48_RS01045 (FOH48_001020)	53263..53613	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOH48_RS01050 (FOH48_001025)	53776..54324	+	549	WP_001061574.1	transcriptional regulator	-
FOH48_RS01055	54498..54665	+	168	WP_015059388.1	hypothetical protein	-
FOH48_RS01060 (FOH48_001030)	54665..54859	+	195	WP_000343597.1	hypothetical protein	-
FOH48_RS01065 (FOH48_001035)	54870..55241	+	372	WP_000516916.1	hypothetical protein	-
FOH48_RS01070 (FOH48_001040)	55234..55704	+	471	WP_001281821.1	hypothetical protein	-
FOH48_RS01075 (FOH48_001045)	55719..56054	-	336	WP_000683476.1	hypothetical protein	-
FOH48_RS01080 (FOH48_001050)	56151..56639	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
FOH48_RS01085 (FOH48_001055)	56642..57139	+	498	WP_000062185.1	hypothetical protein	-
FOH48_RS01090 (FOH48_001060)	57354..58058	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FOH48_RS01095 (FOH48_001065)	58121..58414	-	294	Protein_76	tunicamycin resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / blaNDM-1 / aadA16 / dfrA27 / aac(6')-Ib-cr / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2	-	1..147763	147763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T159620 WP_000270043.1 NZ_CP054402:c53613-53263 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T159620 NZ_CP070098:c816903-816517 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT159620 WP_000124640.1 NZ_CP054402:c53258-52956 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT159620 NZ_CP070098:c816527-816198 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9