Detailed information of TA system TA159612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3745190..3745787
• Replicon: chromosome
• Accession: NZ_CP054400
• Organism: Klebsiella pneumoniae strain KCJ3K307

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: FOH51_RS20160
• Protein ID: WP_004893639.1
• Coordinates: 3745470..3745787 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: FOH51_RS20155
• Protein ID: WP_004142561.1
• Coordinates: 3745190..3745477 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH51_RS20125 (FOH51_020110)	3741270..3741518	+	249	WP_032410665.1	DUF1158 domain-containing protein	-
FOH51_RS20130 (FOH51_020115)	3741536..3741877	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
FOH51_RS20135 (FOH51_020120)	3741908..3743023	-	1116	WP_185967404.1	MBL fold metallo-hydrolase	-
FOH51_RS20140 (FOH51_020125)	3743203..3743784	+	582	WP_032410661.1	TetR/AcrR family transcriptional regulator	-
FOH51_RS20145 (FOH51_020130)	3743784..3744152	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
FOH51_RS20150 (FOH51_020135)	3744272..3744925	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
FOH51_RS20155 (FOH51_020140)	3745190..3745477	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
FOH51_RS20160 (FOH51_020145)	3745470..3745787	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOH51_RS20165 (FOH51_020150)	3745972..3747015	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
FOH51_RS20170 (FOH51_020155)	3747681..3748547	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
FOH51_RS20175 (FOH51_020160)	3748656..3750083	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T159612 WP_004893639.1 NZ_CP054400:c3745787-3745470 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T159612 NZ_CP070092:c2582053-2581835 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT159612 WP_004142561.1 NZ_CP054400:c3745477-3745190 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT159612 NZ_CP070092:c2582454-2582080 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1