Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 312209..312795 Replicon chromosome
Accession NZ_CP054400
Organism Klebsiella pneumoniae strain KCJ3K307

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag FOH51_RS03090 Protein ID WP_002920800.1
Coordinates 312427..312795 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag FOH51_RS03085 Protein ID WP_004174006.1
Coordinates 312209..312430 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOH51_RS03065 (FOH51_003055) 308366..309292 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
FOH51_RS03070 (FOH51_003060) 309289..310566 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
FOH51_RS03075 (FOH51_003065) 310563..311330 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
FOH51_RS03080 (FOH51_003070) 311332..312045 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
FOH51_RS03085 (FOH51_003075) 312209..312430 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FOH51_RS03090 (FOH51_003080) 312427..312795 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
FOH51_RS03095 (FOH51_003085) 313068..314384 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
FOH51_RS03100 (FOH51_003090) 314491..315378 + 888 WP_143873423.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
FOH51_RS03105 (FOH51_003095) 315375..316220 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
FOH51_RS03110 (FOH51_003100) 316222..317292 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 309289..318029 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T159599 WP_002920800.1 NZ_CP054400:312427-312795 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T159599 NZ_CP070088:c3114402-3114118 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT159599 WP_004174006.1 NZ_CP054400:312209-312430 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT159599 NZ_CP070088:c3114634-3114392 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References