Detailed information of TA system TA159598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 135644..136301
• Replicon: plasmid pKC148K
• Accession: NZ_CP054399
• Organism: Klebsiella pneumoniae strain KCJ3K307

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FOH51_RS01510
• Protein ID: WP_000270043.1
• Coordinates: 135951..136301 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOH51_RS01505
• Protein ID: WP_000124640.1
• Coordinates: 135644..135946 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH51_RS01460 (FOH51_001450)	131269..131697	+	429	WP_000591074.1	hypothetical protein	-
FOH51_RS01465 (FOH51_001455)	131754..132113	+	360	WP_000422768.1	hypothetical protein	-
FOH51_RS01470 (FOH51_001460)	132113..132559	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
FOH51_RS01475 (FOH51_001465)	132556..133074	+	519	WP_000210756.1	nitrite reductase	-
FOH51_RS01480 (FOH51_001470)	133074..133304	+	231	WP_000972663.1	hypothetical protein	-
FOH51_RS01485 (FOH51_001475)	133291..134148	+	858	WP_001167032.1	hypothetical protein	-
FOH51_RS01490 (FOH51_001480)	134379..134906	+	528	WP_001236377.1	thermonuclease family protein	-
FOH51_RS01495 (FOH51_001485)	134964..135236	+	273	WP_001043047.1	HU family DNA-binding protein	-
FOH51_RS01500 (FOH51_001490)	135324..135617	+	294	WP_001239997.1	chromosome segregation protein ParM	-
FOH51_RS01505 (FOH51_001495)	135644..135946	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FOH51_RS01510 (FOH51_001500)	135951..136301	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOH51_RS01515 (FOH51_001505)	136464..137012	+	549	WP_001061574.1	transcriptional regulator	-
FOH51_RS01520 (FOH51_001510)	137353..137547	+	195	WP_000343597.1	hypothetical protein	-
FOH51_RS01525 (FOH51_001515)	137558..137929	+	372	WP_000516916.1	hypothetical protein	-
FOH51_RS01530 (FOH51_001520)	137922..138392	+	471	WP_001281821.1	hypothetical protein	-
FOH51_RS01535 (FOH51_001525)	138407..138742	-	336	WP_000683476.1	hypothetical protein	-
FOH51_RS01540 (FOH51_001530)	138839..139327	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
FOH51_RS01545 (FOH51_001535)	139330..139827	+	498	WP_000062185.1	hypothetical protein	-
FOH51_RS01550 (FOH51_001540)	140042..140746	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FOH51_RS01555 (FOH51_001545)	140809..141102	-	294	Protein_170	tunicamycin resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / blaNDM-1 / aadA16 / dfrA27 / aac(6')-Ib-cr / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A)	-	1..147763	147763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T159598 WP_000270043.1 NZ_CP054399:c136301-135951 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T159598 NZ_CP070088:c3023410-3023069 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCTGTGGCAGTCTGGCAAATGCTGCTTGC
TCGCCTGCTGGAACAACATTATGGCCTGACGCTAAACGACACGCCGTTCAGTGATGAAAAGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCTAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT159598 WP_000124640.1 NZ_CP054399:c135946-135644 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT159598 NZ_CP070088:c3023772-3023431 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTAAAATGGGGACTGAATTGTAATATGACACCTTGCTTTGGTGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGATCGGGCCTGCATTACCGGTGAATTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCACACGTTTTATCCGCCAGATGGAATCGATGCTGACTACTGGTGAAATAAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCTCACTCATCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9