Detailed information of TA system TA159591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4839149..4839948
• Replicon: chromosome
• Accession: NZ_CP054388
• Organism: Escherichia coli strain 1517k

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: E2126_RS22975
• Protein ID: WP_000347273.1
• Coordinates: 4839149..4839613 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: E2126_RS22980
• Protein ID: WP_001308975.1
• Coordinates: 4839613..4839948 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2126_RS22945	4834150..4834584	-	435	WP_000948834.1	PTS sugar transporter subunit IIA	-
E2126_RS22950	4834602..4835480	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
E2126_RS22955	4835470..4836249	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
E2126_RS22960	4836260..4836733	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
E2126_RS22965	4836756..4838036	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
E2126_RS22970	4838285..4839094	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
E2126_RS22975	4839149..4839613	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
E2126_RS22980	4839613..4839948	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
E2126_RS22985	4840097..4841668	-	1572	WP_001273756.1	galactarate dehydratase	-
E2126_RS22990	4842043..4843377	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
E2126_RS22995	4843393..4844163	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T159591 WP_000347273.1 NZ_CP054388:c4839613-4839149 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T159591 NZ_CP070088:214396-214728 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT159591 WP_001308975.1 NZ_CP054388:c4839948-4839613 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT159591 NZ_CP070088:214138-214395 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08