Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1619789..1620351 Replicon chromosome
Accession NZ_CP054388
Organism Escherichia coli strain 1517k

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag E2126_RS07600 Protein ID WP_000605676.1
Coordinates 1620073..1620351 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag E2126_RS07595 Protein ID WP_000781370.1
Coordinates 1619789..1620073 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2126_RS07580 1615148..1618195 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
E2126_RS07585 1618208..1619092 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
E2126_RS07590 1619085..1619738 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
E2126_RS07595 1619789..1620073 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
E2126_RS07600 1620073..1620351 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E2126_RS07605 1620537..1621547 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
E2126_RS07610 1621681..1623378 - 1698 WP_000433464.1 malate dehydrogenase -
E2126_RS07615 1623535..1623672 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
E2126_RS07620 1623774..1623989 - 216 WP_000495766.1 biofilm-dependent modulation protein -
E2126_RS07625 1624334..1624765 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T159570 WP_000605676.1 NZ_CP054388:c1620351-1620073 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T159570 NZ_CP070077:4091419-4091670 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT159570 WP_000781370.1 NZ_CP054388:c1620073-1619789 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT159570 NZ_CP070077:4091667-4091921 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References