Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4077411..4078210 Replicon chromosome
Accession NZ_CP054371
Organism Escherichia coli strain SCU-316

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HPE41_RS19160 Protein ID WP_000347273.1
Coordinates 4077746..4078210 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HPE41_RS19155 Protein ID WP_001307405.1
Coordinates 4077411..4077746 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE41_RS19140 4073196..4073966 - 771 WP_001058233.1 2-dehydro-3-deoxyglucarate aldolase -
HPE41_RS19145 4073982..4075316 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HPE41_RS19150 4075691..4077262 + 1572 WP_001273763.1 galactarate dehydratase -
HPE41_RS19155 4077411..4077746 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HPE41_RS19160 4077746..4078210 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HPE41_RS19165 4078265..4079074 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HPE41_RS19170 4079323..4080603 + 1281 WP_000681939.1 tagatose-bisphosphate aldolase subunit KbaZ -
HPE41_RS19175 4080626..4081099 + 474 WP_001626031.1 PTS N-acetylgalactosamine transporter subunit IIB -
HPE41_RS19180 4081110..4081889 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HPE41_RS19185 4081879..4082757 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HPE41_RS19190 4082775..4083209 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T159560 WP_000347273.1 NZ_CP054371:4077746-4078210 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T159560 NZ_CP070077:c1654995-1654822 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT159560 WP_001307405.1 NZ_CP054371:4077411-4077746 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT159560 NZ_CP070077:c1655319-1655023 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References