Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/YafQ-RelB |
| Location | 874441..874982 | Replicon | chromosome |
| Accession | NZ_CP054371 | ||
| Organism | Escherichia coli strain SCU-316 | ||
Toxin (Protein)
| Gene name | yafQ | Uniprot ID | U9XF38 |
| Locus tag | HPE41_RS04095 | Protein ID | WP_001695460.1 |
| Coordinates | 874441..874719 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | S1EWQ0 |
| Locus tag | HPE41_RS04100 | Protein ID | WP_000729704.1 |
| Coordinates | 874722..874982 (-) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HPE41_RS04080 | 872023..872601 | + | 579 | WP_000284050.1 | D-sedoheptulose 7-phosphate isomerase | - |
| HPE41_RS04085 | 872807..873574 | + | 768 | WP_000333379.1 | class II glutamine amidotransferase | - |
| HPE41_RS04090 | 873545..874285 | - | 741 | WP_001225679.1 | murein L,D-transpeptidase | - |
| HPE41_RS04095 | 874441..874719 | - | 279 | WP_001695460.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | Toxin |
| HPE41_RS04100 | 874722..874982 | - | 261 | WP_000729704.1 | type II toxin-antitoxin system antitoxin DinJ | Antitoxin |
| HPE41_RS04105 | 875192..875941 | + | 750 | WP_112033627.1 | C40 family peptidase | - |
| HPE41_RS04110 | 876218..876715 | + | 498 | WP_000006265.1 | REP-associated tyrosine transposase RayT | - |
| HPE41_RS04115 | 876931..878670 | - | 1740 | WP_001312562.1 | flagellar biosynthesis protein FlhA | - |
| HPE41_RS04120 | 878615..879400 | + | 786 | WP_000207556.1 | putative lateral flagellar export/assembly protein LafU | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA | 867474..876715 | 9241 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10830.62 Da Isoelectric Point: 10.0702
>T159539 WP_001695460.1 NZ_CP054371:c874719-874441 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYIMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYIMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
Download Length: 279 bp
>T159539 NZ_CP070071:4082047-4082154 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATAGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATAGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9391.77 Da Isoelectric Point: 5.0758
>AT159539 WP_000729704.1 NZ_CP054371:c874982-874722 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
Download Length: 261 bp
>AT159539 NZ_CP070071:c4081998-4081932 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9XF38 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 4ML0 | |
| AlphaFold DB | A0A0E0Y6Z6 |