Detailed information of TA system TA159531

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 122627..123048
• Replicon: plasmid pSCU-115-1
• Accession: NZ_CP054369
• Organism: Escherichia coli strain SCU-115

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: HPE48_RS24140
• Protein ID: WP_001302184.1
• Coordinates: 122627..122785 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	122850..123048 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE48_RS24100	117692..117907	-	216	WP_000086381.1	conjugal transfer relaxosome protein TraY	-
HPE48_RS24105	118043..118690	-	648	WP_000332519.1	conjugal transfer transcriptional regulator TraJ	-
HPE48_RS24110	118881..119264	-	384	WP_001063021.1	relaxosome protein TraM	-
HPE48_RS24115	119616..120206	+	591	WP_166740857.1	transglycosylase SLT domain-containing protein	-
HPE48_RS24120	120502..121323	-	822	WP_174144237.1	DUF945 domain-containing protein	-
HPE48_RS24125	121442..121729	-	288	WP_000107542.1	hypothetical protein	-
HPE48_RS24130	121811..122029	+	219	Protein_113	hypothetical protein	-
HPE48_RS24135	122030..122389	+	360	Protein_114	hypothetical protein	-
HPE48_RS24140	122627..122785	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	122850..123048	-	199	NuclAT_0	-	Antitoxin
-	122850..123048	-	199	NuclAT_0	-	Antitoxin
-	122850..123048	-	199	NuclAT_0	-	Antitoxin
-	122850..123048	-	199	NuclAT_0	-	Antitoxin
HPE48_RS24145	123060..123779	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
HPE48_RS24150	123776..124210	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
HPE48_RS24155	124265..126223	-	1959	WP_021516762.1	ParB/RepB/Spo0J family partition protein	-
HPE48_RS24160	126289..126522	-	234	WP_000005990.1	DUF905 family protein	-
HPE48_RS24165	126579..127031	-	453	WP_000290802.1	single-stranded DNA-binding protein	-
HPE48_RS24170	127538..127729	-	192	WP_001027500.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / hlyB / iroB / iroC / iroD / iroE / iroN / papC / papH	1..148443	148443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T159531 WP_001302184.1 NZ_CP054369:c122785-122627 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T159531 NZ_CP070071:2044464-2044850 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT159531 NZ_CP054369:c123048-122850 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin