Detailed information of TA system TA159528

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86022..86276
• Replicon: plasmid pSCU-115-1
• Accession: NZ_CP054369
• Organism: Escherichia coli strain SCU-115

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HPE48_RS23930
• Protein ID: WP_001351576.1
• Coordinates: 86022..86228 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	86215..86276 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE48_RS23890	81802..82347	-	546	WP_001523466.1	hypothetical protein	-
HPE48_RS23895	82357..82644	-	288	WP_001523468.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPE48_RS23900	82641..82892	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HPE48_RS23905	82979..83146	-	168	WP_032146603.1	hypothetical protein	-
HPE48_RS23910	83848..84705	-	858	WP_001523472.1	incFII family plasmid replication initiator RepA	-
HPE48_RS23915	84698..85180	-	483	WP_001523473.1	hypothetical protein	-
HPE48_RS23920	85173..85247	-	75	WP_001442103.1	RepA leader peptide Tap	-
HPE48_RS23925	85481..85738	-	258	WP_000083845.1	replication regulatory protein RepA	-
HPE48_RS23930	86022..86228	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	86215..86276	+	62	NuclAT_1	-	Antitoxin
-	86215..86276	+	62	NuclAT_1	-	Antitoxin
-	86215..86276	+	62	NuclAT_1	-	Antitoxin
-	86215..86276	+	62	NuclAT_1	-	Antitoxin
HPE48_RS23935	86859..87071	-	213	WP_032084501.1	hypothetical protein	-
HPE48_RS23940	87204..87764	-	561	WP_001523482.1	fertility inhibition protein FinO	-
HPE48_RS23945	87819..88565	-	747	WP_001523483.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / hlyB / iroB / iroC / iroD / iroE / iroN / papC / papH	1..148443	148443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T159528 WP_001351576.1 NZ_CP054369:c86228-86022 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T159528 NZ_CP070071:c1490999-1490823 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT159528 NZ_CP054369:86215-86276 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin