Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3732080..3732879 Replicon chromosome
Accession NZ_CP054368
Organism Escherichia coli strain SCU-115

Toxin (Protein)


Gene name yhaV Uniprot ID F4T3F3
Locus tag HPE48_RS18150 Protein ID WP_000347257.1
Coordinates 3732080..3732544 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag HPE48_RS18155 Protein ID WP_001296435.1
Coordinates 3732544..3732879 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE48_RS18120 3727081..3727515 - 435 WP_137497329.1 PTS sugar transporter subunit IIA -
HPE48_RS18125 3727533..3728411 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HPE48_RS18130 3728401..3729180 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HPE48_RS18135 3729191..3729664 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HPE48_RS18140 3729687..3730967 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
HPE48_RS18145 3731216..3732025 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HPE48_RS18150 3732080..3732544 - 465 WP_000347257.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HPE48_RS18155 3732544..3732879 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HPE48_RS18160 3733028..3734599 - 1572 WP_001273763.1 galactarate dehydratase -
HPE48_RS18165 3734974..3736308 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HPE48_RS18170 3736324..3737094 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17763.11 Da        Isoelectric Point: 9.4947

>T159524 WP_000347257.1 NZ_CP054368:c3732544-3732080 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVVEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T159524 NZ_CP070071:769976-770191 [Escherichia coli]
CTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGATCAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGA
AGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAGGTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGG
TATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGTCGTTATCATTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT159524 WP_001296435.1 NZ_CP054368:c3732879-3732544 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT159524 NZ_CP070071:769689-769940 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6D0FYC8

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References