Detailed information of TA system TA159519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2901908..2902510
• Replicon: chromosome
• Accession: NZ_CP054368
• Organism: Escherichia coli strain SCU-115

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3L4YH82
• Locus tag: HPE48_RS14135
• Protein ID: WP_000897303.1
• Coordinates: 2902199..2902510 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPE48_RS14130
• Protein ID: WP_000356397.1
• Coordinates: 2901908..2902198 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE48_RS14105	2897836..2898738	+	903	WP_000331383.1	formate dehydrogenase O subunit beta	-
HPE48_RS14110	2898735..2899370	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HPE48_RS14115	2899367..2900296	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
HPE48_RS14120	2900626..2900868	-	243	WP_001086392.1	hypothetical protein	-
HPE48_RS14125	2901086..2901304	-	219	WP_174143972.1	ribbon-helix-helix domain-containing protein	-
HPE48_RS14130	2901908..2902198	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HPE48_RS14135	2902199..2902510	-	312	WP_000897303.1	hypothetical protein	Toxin
HPE48_RS14140	2902739..2903647	+	909	WP_174143973.1	alpha/beta hydrolase	-
HPE48_RS14145	2903711..2904652	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HPE48_RS14150	2904697..2905134	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HPE48_RS14155	2905131..2906000	-	870	WP_021555401.1	virulence factor BrkB family protein	-
HPE48_RS14160	2905994..2906593	-	600	WP_174143974.1	glucose-1-phosphatase	-
HPE48_RS14165	2906692..2907477	-	786	WP_001523571.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12302.33 Da
• Isoelectric Point: 10.0233

>T159519 WP_000897303.1 NZ_CP054368:c2902510-2902199 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGRLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T159519 NZ_CP070069:c3323011-3322760 [Bordetella pertussis]
ATTGGAGGCGCGGCGACAAAAAAGCCGGGACAAAAGCGTCGCGCCGGGGCGGCGGCGCTGGGGCTGGATTTCAGCGCCAT
GGTCGAAATCGTGCTTGCATTGACACCGCGGGATTTCCACAAAAGCATGACTACCCATGCCGACCATCGCATTTGGCAGG
ACGTCTATTGCCCGGTGACAGCGATGGGCGCCGTCTACCTGAAGCTGACGGTCCTCGACGGCGTGCCGATCGTGTCGTTC
AAGGAGTTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT159519 WP_000356397.1 NZ_CP054368:c2902198-2901908 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT159519 NZ_CP070069:c3322757-3322356 [Bordetella pertussis]
ATGAAGTGTCCCGTATGCGGAGCGGCCGCGCTGGCGCGTGATACTCGCGATGTGCCCTATAGCTACAAGGGCGAGACCAT
CACGATTGCGGCGGTGACGGGCGACTGGTGTCCGGCATGCGGCGAGAACGTGCTCGATGGCGCTGAATCGGCGCGGGTGA
GCGAGGCCATGCTGGCTTTCAACCGCCTGGTCAACGCCCGCCGCGTCGACCCCGGGTTCATCGTGCGGGTGCGCAGGAAG
CTGGGGCTGGACCAGCGCGAAGCCGCGGCGCTGTTCGGCGGCGGCACCAATGCGTTCTCGCGTTACGAGAACGGCAAGAC
GCGCCCGCCGCTGGCGCTGGTGAAGCTGCTCAGGCTGCTGGATCGCCATCCGGAGCTGCTGGAGGAAGTCCGTGCGGATT
GA

Structures

Toxin

• AlphaFold DB: A0A3L4YH82