Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1737045..1737724 Replicon chromosome
Accession NZ_CP054368
Organism Escherichia coli strain SCU-115

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag HPE48_RS08755 Protein ID WP_000057523.1
Coordinates 1737422..1737724 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HPE48_RS08750 Protein ID WP_000806442.1
Coordinates 1737045..1737386 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE48_RS08740 1733289..1734221 - 933 WP_001524187.1 glutaminase A -
HPE48_RS08745 1734483..1736987 + 2505 WP_174143820.1 copper-exporting P-type ATPase CopA -
HPE48_RS08750 1737045..1737386 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
HPE48_RS08755 1737422..1737724 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HPE48_RS08760 1737857..1738651 + 795 WP_174143821.1 TraB/GumN family protein -
HPE48_RS08765 1738855..1739334 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HPE48_RS08770 1739371..1741023 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HPE48_RS08775 1741241..1742461 + 1221 WP_096935245.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T159514 WP_000057523.1 NZ_CP054368:c1737724-1737422 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T159514 NZ_CP070066:c4295133-4294756 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT159514 WP_000806442.1 NZ_CP054368:c1737386-1737045 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT159514 NZ_CP070066:c4295591-4295223 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References