Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 800502..801064 Replicon chromosome
Accession NZ_CP054368
Organism Escherichia coli strain SCU-115

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HPE48_RS04190 Protein ID WP_000605675.1
Coordinates 800502..800780 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HPE48_RS04195 Protein ID WP_000781370.1
Coordinates 800780..801064 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE48_RS04165 796090..796521 - 432 WP_174143735.1 peroxiredoxin OsmC -
HPE48_RS04170 796865..797080 + 216 WP_000495774.1 biofilm-dependent modulation protein -
HPE48_RS04175 797182..797319 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HPE48_RS04180 797475..799172 + 1698 WP_000433462.1 malate dehydrogenase -
HPE48_RS04185 799306..800316 + 1011 WP_000642417.1 alcohol dehydrogenase AdhP -
HPE48_RS04190 800502..800780 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HPE48_RS04195 800780..801064 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HPE48_RS04200 801293..801946 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HPE48_RS04205 801939..802823 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HPE48_RS04210 802836..805883 - 3048 WP_174143736.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T159513 WP_000605675.1 NZ_CP054368:800502-800780 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T159513 NZ_CP070066:4155374-4155526 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT159513 WP_000781370.1 NZ_CP054368:800780-801064 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT159513 NZ_CP070066:c4155325-4155268 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References