Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 19673..20319 Replicon plasmid pSCU-171-2
Accession NZ_CP054365
Organism Escherichia coli strain SCU-171

Toxin (Protein)


Gene name higB Uniprot ID E9TBD5
Locus tag HPE49_RS26340 Protein ID WP_000269927.1
Coordinates 19969..20319 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID E9TBD4
Locus tag HPE49_RS26335 Protein ID WP_001259442.1
Coordinates 19673..19972 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE49_RS26295 15046..15354 + 309 WP_000447215.1 hypothetical protein -
HPE49_RS26300 15420..16070 + 651 WP_096950303.1 hypothetical protein -
HPE49_RS26305 16280..16765 + 486 WP_001061740.1 hypothetical protein -
HPE49_RS26310 17339..17563 + 225 WP_050418674.1 hypothetical protein -
HPE49_RS26315 17596..17946 + 351 WP_000694584.1 hypothetical protein -
HPE49_RS26320 18266..18889 + 624 WP_001127156.1 ParA family protein -
HPE49_RS26325 18886..19095 + 210 WP_000041404.1 hypothetical protein -
HPE49_RS26330 19124..19615 + 492 WP_000456858.1 hypothetical protein -
HPE49_RS26335 19673..19972 - 300 WP_001259442.1 XRE family transcriptional regulator Antitoxin
HPE49_RS26340 19969..20319 - 351 WP_000269927.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HPE49_RS26345 20677..21180 + 504 WP_021526598.1 hypothetical protein -
HPE49_RS26350 21349..21717 + 369 WP_001330915.1 hypothetical protein -
HPE49_RS26355 22285..22881 - 597 WP_021528077.1 hypothetical protein -
HPE49_RS26360 22878..23405 - 528 WP_001330917.1 hypothetical protein -
HPE49_RS26365 23417..23632 + 216 WP_001280801.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..31162 31162


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13770.78 Da        Isoelectric Point: 7.4219

>T159500 WP_000269927.1 NZ_CP054365:c20319-19969 [Escherichia coli]
MWTVYFGRLFDEWFEEQELALKRKVLAELRHLEEFGPSLSRPHADTVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAI
ILCAGDKSNDKKFYDRMIRIADEEFSTHLAELEGKK

Download         Length: 351 bp

>T159500 NZ_CP070066:310575-310682 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10931.73 Da        Isoelectric Point: 4.6283

>AT159500 WP_001259442.1 NZ_CP054365:c19972-19673 [Escherichia coli]
MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQKQVADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp

>AT159500 NZ_CP070066:c310518-310461 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9TBD5

Antitoxin

Source ID Structure
AlphaFold DB E9TBD4

References