Detailed information of TA system TA159489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3058499..3059101
• Replicon: chromosome
• Accession: NZ_CP054363
• Organism: Escherichia coli strain SCU-171

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HPE49_RS14685
• Protein ID: WP_000897305.1
• Coordinates: 3058499..3058810 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPE49_RS14690
• Protein ID: WP_000356397.1
• Coordinates: 3058811..3059101 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE49_RS14660	3054413..3055012	+	600	WP_001314324.1	glucose-1-phosphatase	-
HPE49_RS14665	3055006..3055878	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HPE49_RS14670	3055875..3056312	+	438	WP_021560109.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HPE49_RS14675	3056357..3057298	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HPE49_RS14680	3057362..3058270	-	909	WP_001318161.1	alpha/beta hydrolase	-
HPE49_RS14685	3058499..3058810	+	312	WP_000897305.1	hypothetical protein	Toxin
HPE49_RS14690	3058811..3059101	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HPE49_RS14695	3059706..3059924	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
HPE49_RS14700	3060139..3061068	-	930	WP_052920312.1	formate dehydrogenase accessory protein FdhE	-
HPE49_RS14705	3061065..3061700	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HPE49_RS14710	3061697..3062599	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T159489 WP_000897305.1 NZ_CP054363:3058499-3058810 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T159489 NZ_CP070063:c3057301-3057199 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT159489 WP_000356397.1 NZ_CP054363:3058811-3059101 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT159489 NZ_CP070063:3057161-3057429 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7