Detailed information of TA system TA159466

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74531..74784
• Replicon: plasmid pSCU-172-3
• Accession: NZ_CP054356
• Organism: Escherichia coli strain SCU-172

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: HPE50_RS26850
• Protein ID: WP_001336447.1
• Coordinates: 74635..74784 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	74531..74587 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE50_RS26820	70443..71189	+	747	WP_000205726.1	conjugal transfer pilus acetylase TraX	-
HPE50_RS26825	71244..71801	+	558	WP_000139329.1	fertility inhibition protein FinO	-
HPE50_RS26830	71953..72156	+	204	WP_001336517.1	hypothetical protein	-
HPE50_RS26835	72898..73359	+	462	WP_019842128.1	thermonuclease family protein	-
HPE50_RS26840	73628..73846	+	219	WP_023150739.1	hypothetical protein	-
HPE50_RS26845	73999..74436	+	438	WP_000872609.1	hypothetical protein	-
-	74531..74587	-	57	NuclAT_1	-	Antitoxin
-	74531..74587	-	57	NuclAT_1	-	Antitoxin
-	74531..74587	-	57	NuclAT_1	-	Antitoxin
-	74531..74587	-	57	NuclAT_1	-	Antitoxin
HPE50_RS26850	74635..74784	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
HPE50_RS26855	75069..75326	+	258	WP_000084404.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..75628	75628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T159466 WP_001336447.1 NZ_CP054356:74635-74784 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T159466 NZ_CP070048:4168814-4168987 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 57 bp

>AT159466 NZ_CP054356:c74587-74531 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin