Detailed information of TA system TA159463

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34200..34469
• Replicon: plasmid pSCU-172-3
• Accession: NZ_CP054356
• Organism: Escherichia coli strain SCU-172

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HPE50_RS26615
• Protein ID: WP_001312861.1
• Coordinates: 34311..34469 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34200..34265 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE50_RS26580	29668..29904	-	237	WP_032072846.1	hypothetical protein	-
HPE50_RS26585	29969..30496	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HPE50_RS26590	30554..30787	+	234	WP_000006003.1	DUF905 family protein	-
HPE50_RS26595	30848..32812	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
HPE50_RS26600	32881..33315	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HPE50_RS26605	33312..34031	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	34043..34267	+	225	NuclAT_0	-	-
-	34043..34267	+	225	NuclAT_0	-	-
-	34043..34267	+	225	NuclAT_0	-	-
-	34043..34267	+	225	NuclAT_0	-	-
HPE50_RS26610	34052..34231	-	180	WP_001309233.1	hypothetical protein	-
-	34200..34265	+	66	-	-	Antitoxin
HPE50_RS26615	34311..34469	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HPE50_RS26620	34707..35085	-	379	Protein_43	hypothetical protein	-
HPE50_RS26625	35381..35677	+	297	WP_001272251.1	hypothetical protein	-
HPE50_RS26630	35788..36609	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HPE50_RS26635	36906..37508	-	603	Protein_46	transglycosylase SLT domain-containing protein	-
HPE50_RS26640	37829..38212	+	384	WP_001151538.1	relaxosome protein TraM	-
HPE50_RS26645	38399..39088	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..75628	75628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T159463 WP_001312861.1 NZ_CP054356:34311-34469 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T159463 NZ_CP070048:c2985913-2985599 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAATCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT159463 NZ_CP054356:34200-34265 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin