Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4498423..4499222 Replicon chromosome
Accession NZ_CP054353
Organism Escherichia coli strain SCU-172

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HPE50_RS22250 Protein ID WP_000347273.1
Coordinates 4498758..4499222 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A210GLR0
Locus tag HPE50_RS22245 Protein ID WP_001571956.1
Coordinates 4498423..4498758 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE50_RS22225 4493431..4494201 - 771 WP_001058217.1 2-dehydro-3-deoxyglucarate aldolase -
HPE50_RS22230 4494217..4495551 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HPE50_RS22235 4496018..4496715 - 698 Protein_4335 IS1 family transposase -
HPE50_RS22240 4496772..4498274 + 1503 Protein_4336 galactarate dehydratase -
HPE50_RS22245 4498423..4498758 + 336 WP_001571956.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HPE50_RS22250 4498758..4499222 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HPE50_RS22255 4499277..4500086 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HPE50_RS22260 4500335..4501615 + 1281 WP_000681958.1 tagatose-bisphosphate aldolase subunit KbaZ -
HPE50_RS22265 4501638..4502111 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HPE50_RS22270 4502122..4502901 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HPE50_RS22275 4502891..4503769 + 879 WP_174183276.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HPE50_RS22280 4503787..4504221 + 435 WP_000948834.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4487277..4499222 11945
- flank IS/Tn - - 4496018..4496521 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T159458 WP_000347273.1 NZ_CP054353:4498758-4499222 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T159458 NZ_CP070048:c1710901-1710647 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12328.96 Da        Isoelectric Point: 4.6647

>AT159458 WP_001571956.1 NZ_CP054353:4498423-4498758 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQITRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT159458 NZ_CP070048:c1711149-1710898 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A210GLR0

References