Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1918676..1919178 Replicon chromosome
Accession NZ_CP054353
Organism Escherichia coli strain SCU-172

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag HPE50_RS09260 Protein ID WP_000767828.1
Coordinates 1918924..1919178 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HPE50_RS09255 Protein ID WP_001259255.1
Coordinates 1918676..1918927 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE50_RS09230 1913854..1914921 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HPE50_RS09235 1914921..1915991 - 1071 WP_000108947.1 histidinol-phosphate transaminase -
HPE50_RS09240 1915988..1917292 - 1305 WP_001350335.1 histidinol dehydrogenase -
HPE50_RS09245 1917298..1918197 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HPE50_RS09250 1918343..1918393 - 51 WP_001364200.1 his operon leader peptide -
HPE50_RS09255 1918676..1918927 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HPE50_RS09260 1918924..1919178 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HPE50_RS09265 1919261..1920085 + 825 WP_000754740.1 SDR family oxidoreductase -
HPE50_RS09270 1920131..1921060 + 930 WP_000803351.1 LysR family transcriptional regulator -
HPE50_RS09275 1921275..1921337 + 63 WP_010723108.1 membrane protein YoeI -
HPE50_RS09280 1921327..1922685 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HPE50_RS09285 1922864..1923922 + 1059 WP_000492324.1 transport protein YeeE -
HPE50_RS09290 1923936..1924163 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T159442 WP_000767828.1 NZ_CP054353:1918924-1919178 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T159442 NZ_CP070046:2833338-2833622 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT159442 WP_001259255.1 NZ_CP054353:1918676-1918927 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT159442 NZ_CP070046:2833106-2833348 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGTGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References