Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1085797..1086491 Replicon chromosome
Accession NZ_CP054353
Organism Escherichia coli strain SCU-172

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag HPE50_RS05180 Protein ID WP_001263491.1
Coordinates 1086093..1086491 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag HPE50_RS05175 Protein ID WP_000554755.1
Coordinates 1085797..1086090 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE50_RS05150 1081611..1082078 + 468 WP_000725257.1 flagellar basal body-associated FliL family protein -
HPE50_RS05155 1082098..1082817 + 720 WP_000938736.1 FliA/WhiG family RNA polymerase sigma factor -
HPE50_RS05160 1082830..1083693 + 864 WP_001169516.1 flagellar motor stator protein MotA -
HPE50_RS05165 1083696..1084619 + 924 WP_001570971.1 putative lateral flagellar export/assembly protein LafU -
HPE50_RS05170 1084690..1085745 + 1056 WP_001226154.1 DNA polymerase IV -
HPE50_RS05175 1085797..1086090 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HPE50_RS05180 1086093..1086491 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HPE50_RS05185 1086501..1086953 + 453 WP_001059882.1 GNAT family N-acetyltransferase -
HPE50_RS05190 1087132..1088280 + 1149 WP_077775519.1 RNA ligase RtcB family protein -
HPE50_RS05195 1088277..1088891 + 615 WP_000602125.1 peptide chain release factor H -
HPE50_RS05200 1088948..1090405 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
HPE50_RS05205 1090666..1091124 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T159438 WP_001263491.1 NZ_CP054353:1086093-1086491 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T159438 NZ_CP070046:c1905363-1905016 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGACGATTACTATGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT159438 WP_000554755.1 NZ_CP054353:1085797-1086090 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT159438 NZ_CP070046:c1905016-1904717 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References