Detailed information of TA system TA159431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4995091..4995693
• Replicon: chromosome
• Accession: NZ_CP054345
• Organism: Escherichia coli strain SCU-176

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HPE52_RS24035
• Protein ID: WP_000897302.1
• Coordinates: 4995091..4995402 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPE52_RS24040
• Protein ID: WP_000356397.1
• Coordinates: 4995403..4995693 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE52_RS24005	4990121..4990906	+	786	WP_097430631.1	DeoR/GlpR family DNA-binding transcription regulator	-
HPE52_RS24010	4991005..4991604	+	600	WP_001314324.1	glucose-1-phosphatase	-
HPE52_RS24015	4991598..4992470	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HPE52_RS24020	4992467..4992904	+	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HPE52_RS24025	4992949..4993890	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HPE52_RS24030	4993954..4994862	-	909	WP_097430632.1	alpha/beta hydrolase	-
HPE52_RS24035	4995091..4995402	+	312	WP_000897302.1	hypothetical protein	Toxin
HPE52_RS24040	4995403..4995693	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HPE52_RS24045	4996052..4996330	+	279	WP_001296612.1	hypothetical protein	-
HPE52_RS24050	4996726..4996944	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HPE52_RS24055	4997160..4998089	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HPE52_RS24060	4998086..4998721	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HPE52_RS24065	4998718..4999620	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T159431 WP_000897302.1 NZ_CP054345:4995091-4995402 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T159431 NZ_CP070045:4422237-4422548 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT159431 WP_000356397.1 NZ_CP054345:4995403-4995693 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT159431 NZ_CP070045:4422549-4422839 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416