Detailed information of TA system TA159422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3367538..3368232
• Replicon: chromosome
• Accession: NZ_CP054345
• Organism: Escherichia coli strain SCU-176

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HPE52_RS16225
• Protein ID: WP_001263500.1
• Coordinates: 3367538..3367936 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HPE52_RS16230
• Protein ID: WP_000554758.1
• Coordinates: 3367939..3368232 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE52_RS16200	3362903..3363361	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
HPE52_RS16205	3363622..3365079	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HPE52_RS16210	3365136..3365750	-	615	WP_000602124.1	peptide chain release factor H	-
HPE52_RS16215	3365747..3366898	-	1152	WP_097430544.1	RNA ligase RtcB family protein	-
HPE52_RS16220	3367076..3367528	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HPE52_RS16225	3367538..3367936	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HPE52_RS16230	3367939..3368232	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HPE52_RS16235	3368284..3369339	-	1056	WP_001226168.1	DNA polymerase IV	-
HPE52_RS16240	3369410..3370333	-	924	WP_115766527.1	putative lateral flagellar export/assembly protein LafU	-
HPE52_RS16245	3370336..3371199	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
HPE52_RS16250	3371212..3371928	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
HPE52_RS16255	3371948..3372415	-	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T159422 WP_001263500.1 NZ_CP054345:c3367936-3367538 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T159422 NZ_CP070045:c1972290-1972188 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT159422 WP_000554758.1 NZ_CP054345:c3368232-3367939 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT159422 NZ_CP070045:1972150-1972418 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3