Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3367538..3368232 Replicon chromosome
Accession NZ_CP054345
Organism Escherichia coli strain SCU-176

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag HPE52_RS16225 Protein ID WP_001263500.1
Coordinates 3367538..3367936 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HPE52_RS16230 Protein ID WP_000554758.1
Coordinates 3367939..3368232 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE52_RS16200 3362903..3363361 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
HPE52_RS16205 3363622..3365079 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
HPE52_RS16210 3365136..3365750 - 615 WP_000602124.1 peptide chain release factor H -
HPE52_RS16215 3365747..3366898 - 1152 WP_097430544.1 RNA ligase RtcB family protein -
HPE52_RS16220 3367076..3367528 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
HPE52_RS16225 3367538..3367936 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HPE52_RS16230 3367939..3368232 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HPE52_RS16235 3368284..3369339 - 1056 WP_001226168.1 DNA polymerase IV -
HPE52_RS16240 3369410..3370333 - 924 WP_115766527.1 putative lateral flagellar export/assembly protein LafU -
HPE52_RS16245 3370336..3371199 - 864 WP_001169518.1 flagellar motor stator protein MotA -
HPE52_RS16250 3371212..3371928 - 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
HPE52_RS16255 3371948..3372415 - 468 WP_000725261.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T159422 WP_001263500.1 NZ_CP054345:c3367936-3367538 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T159422 NZ_CP070045:c1972290-1972188 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT159422 WP_000554758.1 NZ_CP054345:c3368232-3367939 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT159422 NZ_CP070045:1972150-1972418 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References