Detailed information of TA system TA159420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3139798..3140477
• Replicon: chromosome
• Accession: NZ_CP054345
• Organism: Escherichia coli strain SCU-176

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: HPE52_RS15190
• Protein ID: WP_000057523.1
• Coordinates: 3140175..3140477 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: HPE52_RS15185
• Protein ID: WP_000806442.1
• Coordinates: 3139798..3140139 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE52_RS15175	3136042..3136974	-	933	WP_001538399.1	glutaminase A	-
HPE52_RS15180	3137236..3139740	+	2505	WP_000083948.1	copper-exporting P-type ATPase CopA	-
HPE52_RS15185	3139798..3140139	-	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
HPE52_RS15190	3140175..3140477	-	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPE52_RS15195	3140610..3141404	+	795	WP_001538398.1	TraB/GumN family protein	-
HPE52_RS15200	3141608..3142087	+	480	WP_001538397.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HPE52_RS15205	3142124..3143776	-	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HPE52_RS15210	3143994..3145214	+	1221	WP_001251634.1	fosmidomycin MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T159420 WP_000057523.1 NZ_CP054345:c3140477-3140175 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T159420 NZ_CP070045:c1620869-1620591 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT159420 WP_000806442.1 NZ_CP054345:c3140139-3139798 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT159420 NZ_CP070045:c1620591-1620307 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3